OBSCN
OBSCN (англ. Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF) – білок, який кодується однойменним геном, розташованим у людей на короткому плечі 1-ї хромосоми. [3] Довжина поліпептидного ланцюга білка становить 7 968 амінокислот, а молекулярна маса — 868 484[4].
Послідовність амінокислот
10 | 20 | 30 | 40 | 50 | ||||
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MDQPQFSGAP | RFLTRPKAFV | VSVGKDATLS | CQIVGNPTPQ | VSWEKDQQPV | ||||
AAGARFRLAQ | DGDLYRLTIL | DLALGDSGQY | VCRARNAIGE | AFAAVGLQVD | ||||
AEAACAEQAP | HFLLRPTSIR | VREGSEATFR | CRVGGSPRPA | VSWSKDGRRL | ||||
GEPDGPRVRV | EELGEASALR | IRAARPRDGG | TYEVRAENPL | GAASAAAALV | ||||
VDSDAADTAS | RPGTSTAALL | AHLQRRREAM | RAEGAPASPP | STGTRTCTVT | ||||
EGKHARLSCY | VTGEPKPETV | WKKDGQLVTE | GRRHVVYEDA | QENFVLKILF | ||||
CKQSDRGLYT | CTASNLVGQT | YSSVLVVVRE | PAVPFKKRLQ | DLEVREKESA | ||||
TFLCEVPQPS | TEAAWFKEET | RLWASAKYGI | EEEGTERRLT | VRNVSADDDA | ||||
VYICETPEGS | RTVAELAVQG | NLLRKLPRKT | AVRVGDTAMF | CVELAVPVGP | ||||
VHWLRNQEEV | VAGGRVAISA | EGTRHTLTIS | QCCLEDVGQV | AFMAGDCQTS | ||||
TQFCVSAPRK | PPLQPPVDPV | VKARMESSVI | LSWSPPPHGE | RPVTIDGYLV | ||||
EKKKLGTYTW | IRCHEAEWVA | TPELTVADVA | EEGNFQFRVS | ALNSFGQSPY | ||||
LEFPGTVHLA | PKLAVRTPLK | AVQAVEGGEV | TFSVDLTVAS | AGEWFLDGQA | ||||
LKASSVYEIH | CDRTRHTLTI | REVPASLHGA | QLKFVANGIE | SSIRMEVRAA | ||||
PGLTANKPPA | AAAREVLARL | HEEAQLLAEL | SDQAAAVTWL | KDGRTLSPGP | ||||
KYEVQASAGR | RVLLVRDVAR | DDAGLYECVS | RGGRIAYQLS | VQGLARFLHK | ||||
DMAGSCVDAV | AGGPAQFECE | TSEAHVHVHW | YKDGMELGHS | GERFLQEDVG | ||||
TRHRLVAATV | TRQDEGTYSC | RVGEDSVDFR | LRVSEPKVVF | AKEQLARRKL | ||||
QAEAGASATL | SCEVAQAQTE | VTWYKDGKKL | SSSSKVCMEA | TGCTRRLVVQ | ||||
QAGQADAGEY | SCEAGGQRLS | FHLDVKEPKV | VFAKDQVAHS | EVQAEAGASA | ||||
TLSCEVAQAQ | TEVMWYKDGK | KLSSSLKVHV | EAKGCRRRLV | VQQAGKTDAG | ||||
DYSCEARGQR | VSFRLHITEP | KMMFAKEQSV | HNEVQAEAGA | SAMLSCEVAQ | ||||
AQTEVTWYKD | GKKLSSSSKV | GMEVKGCTRR | LVLPQAGKAD | AGEYSCEAGG | ||||
QRVSFHLHIT | EPKGVFAKEQ | SVHNEVQAEA | GTTAMLSCEV | AQPQTEVTWY | ||||
KDGKKLSSSS | KVRMEVKGCT | RRLVVQQVGK | ADAGEYSCEA | GGQRVSFQLH | ||||
ITEPKAVFAK | EQLVHNEVRT | EAGASATLSC | EVAQAQTEVT | WYKDGKKLSS | ||||
SSKVRIEAAG | CMRQLVVQQA | GQADAGEYTC | EAGGQRLSFH | LDVSEPKAVF | ||||
AKEQLAHRKV | QAEAGAIATL | SCEVAQAQTE | VTWYKDGKKL | SSSSKVRMEA | ||||
VGCTRRLVVQ | QACQADTGEY | SCEAGGQRLS | FSLDVAEPKV | VFAKEQPVHR | ||||
EVQAQAGAST | TLSCEVAQAQ | TEVMWYKDGK | KLSFSSKVRM | EAVGCTRRLV | ||||
VQQAGQAVAG | EYSCEAGSQR | LSFHLHVAEP | KAVFAKEQPA | SREVQAEAGT | ||||
SATLSCEVAQ | AQTEVTWYKD | GKKLSSSSKV | RMEAVGCTRR | LVVQEAGQAD | ||||
AGEYSCKAGD | QRLSFHLHVA | EPKVVFAKEQ | PAHREVQAEA | GASATLSCEV | ||||
AQAQTEVTWY | KDGKKLSSSS | KVRVEAVGCT | RRLVVQQAGQ | AEAGEYSCEA | ||||
GGQQLSFRLQ | VAELEPQISE | RPCRREPLVV | KEHEDIILTA | TLATPSAATV | ||||
TWLKDGVEIR | RSKRHETASQ | GDTHTLTVHG | AQVLDSAIYS | CRVGAEGQDF | ||||
PVQVEEVAAK | FCRLLEPVCG | ELGGTVTLAC | ELSPACAEVV | WRCGNTQLRV | ||||
GKRFQMVAEG | PVRSLTVLGL | RAEDAGEYVC | ESRDDHTSAQ | LTVSVPRVVK | ||||
FMSGLSTVVA | EEGGEATFQC | VVSPSDVAVV | WFRDGALLQP | SEKFAISQSG | ||||
ASHSLTISDL | VLEDAGQITV | EAEGASSSAA | LRVREAPVLF | KKKLEPQTVE | ||||
ERSSVTLEVE | LTRPWPELRW | TRNATALAPG | KNVEIHAEGA | RHRLVLHNVG | ||||
FADRGFFGCE | TPDDKTQAKL | TVEMRQVRLV | RGLQAVEARE | QGTATMEVQL | ||||
SHADVDGSWT | RDGLRFQQGP | TCHLAVRGPM | HTLTLSGLRP | EDSGLMVFKA | ||||
EGVHTSARLV | VTELPVSFSR | PLQDVVTTEK | EKVTLECELS | RPNVDVRWLK | ||||
DGVELRAGKT | MAIAAQGACR | SLTIYRCEFA | DQGVYVCDAH | DAQSSASVKV | ||||
QGRTYTLIYR | RVLAEDAGEI | QFVAENAESR | AQLRVKELPV | TLVRPLRDKI | ||||
AMEKHRGVLE | CQVSRASAQV | RWFKGSQELQ | PGPKYELVSD | GLYRKLIISD | ||||
VHAEDEDTYT | CDAGDVKTSA | QFFVEEQSIT | IVRGLQDVTV | MEPAPAWFEC | ||||
ETSIPSVRPP | KWLLGKTVLQ | AGGNVGLEQE | GTVHRLMLRR | TCSTMTGPVH | ||||
FTVGKSRSSA | RLVVSDIPVV | LTRPLEPKTG | RELQSVVLSC | DFRPAPKAVQ | ||||
WYKDDTPLSP | SEKFKMSLEG | QMAELRILRL | MPADAGVYRC | QAGSAHSSTE | ||||
VTVEAREVTV | TGPLQDAEAT | EEGWASFSCE | LSHEDEEVEW | SLNGMPLYND | ||||
SFHEISHKGR | RHTLVLKSIQ | RADAGIVRAS | SLKVSTSARL | EVRVKPVVFL | ||||
KALDDLSAEE | RGTLALQCEV | SDPEAHVVWR | KDGVQLGPSD | KYDFLHTAGT | ||||
RGLVVHDVSP | EDAGLYTCHV | GSEETRARVR | VHDLHVGITK | RLKTMEVLEG | ||||
ESCSFECVLS | HESASDPAMW | TVGGKTVGSS | SRFQATRQGR | KYILVVREAA | ||||
PSDAGEVVFS | VRGLTSKASL | IVRERPAAII | KPLEDQWVAP | GEDVELRCEL | ||||
SRAGTPVHWL | KDRKAIRKSQ | KYDVVCEGTM | AMLVIRGASL | KDAGEYTCEV | ||||
EASKSTASLH | VEEKANCFTE | ELTNLQVEEK | GTAVFTCKTE | HPAATVTWRK | ||||
GLLELRASGK | HQPSQEGLTL | RLTISALEKA | DSDTYTCDIG | QAQSRAQLLV | ||||
QGRRVHIIED | LEDVDVQEGS | SATFRCRISP | ANYEPVHWFL | DKTPLHANEL | ||||
NEIDAQPGGY | HVLTLRQLAL | KDSGTIYFEA | GDQRASAALR | VTEKPSVFSR | ||||
ELTDATITEG | EDLTLVCETS | TCDIPVCWTK | DGKTLRGSAR | CQLSHEGHRA | ||||
QLLITGATLQ | DSGRYKCEAG | GACSSSIVRV | HARPVRFQEA | LKDLEVLEGG | ||||
AATLRCVLSS | VAAPVKWCYG | NNVLRPGDKY | SLRQEGAMLE | LVVRNLRPQD | ||||
SGRYSCSFGD | QTTSATLTVT | ALPAQFIGKL | RNKEATEGAT | ATLRCELSKA | ||||
APVEWRKGSE | TLRDGDRYCL | RQDGAMCELQ | IRGLAMVDAA | EYSCVCGEER | ||||
TSASLTIRPM | PAHFIGRLRH | QESIEGATAT | LRCELSKAAP | VEWRKGRESL | ||||
RDGDRHSLRQ | DGAVCELQIC | GLAVADAGEY | SCVCGEERTS | ATLTVKALPA | ||||
KFTEGLRNEE | AVEGATAMLW | CELSKVAPVE | WRKGPENLRD | GDRYILRQEG | ||||
TRCELQICGL | AMADAGEYLC | VCGQERTSAT | LTIRALPARF | IEDVKNQEAR | ||||
EGATAVLQCE | LNSAAPVEWR | KGSETLRDGD | RYSLRQDGTK | CELQIRGLAM | ||||
ADTGEYSCVC | GQERTSAMLT | VRALPIKFTE | GLRNEEATEG | ATAVLRCELS | ||||
KMAPVEWWKG | HETLRDGDRH | SLRQDGARCE | LQIRGLVAED | AGEYLCMCGK | ||||
ERTSAMLTVR | AMPSKFIEGL | RNEEATEGDT | ATLWCELSKA | APVEWRKGHE | ||||
TLRDGDRHSL | RQDGSRCELQ | IRGLAVVDAG | EYSCVCGQER | TSATLTVRAL | ||||
PARFIEDVKN | QEAREGATAV | LQCELSKAAP | VEWRKGSETL | RGGDRYSLRQ | ||||
DGTRCELQIH | GLSVADTGEY | SCVCGQERTS | ATLTVRAPQP | VFREPLQSLQ | ||||
AEEGSTATLQ | CELSEPTATV | VWSKGGLQLQ | ANGRREPRLQ | GCTAELVLQD | ||||
LQREDTGEYT | CTCGSQATSA | TLTVTAAPVR | FLRELQHQEV | DEGGTAHLCC | ||||
ELSRAGASVE | WRKGSLQLFP | CAKYQMVQDG | AAAELLVRGV | EQEDAGDYTC | ||||
DTGHTQSMAS | LSVRVPRPKF | KTRLQSLEQE | TGDIARLCCQ | LSDAESGAVV | ||||
QWLKEGVELH | AGPKYEMRSQ | GATRELLIHQ | LEAKDTGEYA | CVTGGQKTAA | ||||
SLRVTEPEVT | IVRGLVDAEV | TADEDVEFSC | EVSRAGATGV | QWCLQGLPLQ | ||||
SNEVTEVAVR | DGRIHTLRLK | GVTPEDAGTV | SFHLGNHASS | AQLTVRAPEV | ||||
TILEPLQDVQ | LSEGQDASFQ | CRLSRASGQE | ARWALGGVPL | QANEMNDITV | ||||
EQGTLHLLTL | HKVTLEDAGT | VSFHVGTCSS | EAQLKVTAKN | TVVRGLENVE | ||||
ALEGGEALFE | CQLSQPEVAA | HTWLLDDEPV | HTSENAEVVF | FENGLRHLLL | ||||
LKNLRPQDSC | RVTFLAGDMV | TSAFLTVRGW | RLEILEPLKN | AAVRAGAQAC | ||||
FTCTLSEAVP | VGEASWYING | AAVQPDDSDW | TVTADGSHHA | LLLRSAQPHH | ||||
AGEVTFACRD | AVASARLTVL | GLPDPPEDAE | VVARSSHTVT | LSWAAPMSDG | ||||
GGGLCGYRVE | VKEGATGQWR | LCHELVPGPE | CVVDGLAPGE | TYRFRVAAVG | ||||
PVGAGEPVHL | PQTVRLAEPP | KPVPPQPSAP | ESRQVAAGED | VSLELEVVAE | ||||
AGEVIWHKGM | ERIQPGGRFE | VVSQGRQQML | VIKGFTAEDQ | GEYHCGLAQG | ||||
SICPAAATFQ | VALSPASVDE | APQPSLPPEA | AQEGDLHLLW | EALARKRRMS | ||||
REPTLDSISE | LPEEDGRSQR | LPQEAEEVAP | DLSEGYSTAD | ELARTGDADL | ||||
SHTSSDDESR | AGTPSLVTYL | KKAGRPGTSP | LASKVGAPAA | PSVKPQQQQE | ||||
PLAAVRPPLG | DLSTKDLGDP | SMDKAAVKIQ | AAFKGYKVRK | EMKQQEGPMF | ||||
SHTFGDTEAQ | VGDALRLECV | VASKADVRAR | WLKDGVELTD | GRHHHIDQLG | ||||
DGTCSLLITG | LDRADAGCYT | CQVSNKFGQV | THSACVVVSG | SESEAESSSG | ||||
GELDDAFRRA | ARRLHRLFRT | KSPAEVSDEE | LFLSADEGPA | EPEEPADWQT | ||||
YREDEHFICI | RFEALTEARQ | AVTRFQEMFA | TLGIGVEIKL | VEQGPRRVEM | ||||
CISKETPAPV | VPPEPLPSLL | TSDAAPVFLT | ELQNQEVQDG | YPVSFDCVVT | ||||
GQPMPSVRWF | KDGKLLEEDD | HYMINEDQQG | GHQLIITAVV | PADMGVYRCL | ||||
AENSMGVSST | KAELRVDLTS | TDYDTAADAT | ESSSYFSAQG | YLSSREQEGT | ||||
ESTTDEGQLP | QVVEELRDLQ | VAPGTRLAKF | QLKVKGYPAP | RLYWFKDGQP | ||||
LTASAHIRMT | DKKILHTLEI | ISVTREDSGQ | YAAYISNAMG | AAYSSARLLV | ||||
RGPDEPEEKP | ASDVHEQLVP | PRMLERFTPK | KVKKGSSITF | SVKVEGRPVP | ||||
TVHWLREEAE | RGVLWIGPDT | PGYTVASSAQ | QHSLVLLDVG | RQHQGTYTCI | ||||
ASNAAGQALC | SASLHVSGLP | KVEEQEKVKE | ALISTFLQGT | TQAISAQGLE | ||||
TASFADLGGQ | RKEEPLAAKE | ALGHLSLAEV | GTEEFLQKLT | SQITEMVSAK | ||||
ITQAKLQVPG | GDSDEDSKTP | SASPRHGRSR | PSSSIQESSS | ESEDGDARGE | ||||
IFDIYVVTAD | YLPLGAEQDA | ITLREGQYVE | VLDAAHPLRW | LVRTKPTKSS | ||||
PSRQGWVSPA | YLDRRLKLSP | EWGAAEAPEF | PGEAVSEDEY | KARLSSVIQE | ||||
LLSSEQAFVE | ELQFLQSHHL | QHLERCPHVP | IAVAGQKAVI | FRNVRDIGRF | ||||
HSSFLQELQQ | CDTDDDVAMC | FIKNQAAFEQ | YLEFLVGRVQ | AESVVVSTAI | ||||
QEFYKKYAEE | ALLAGDPSQP | PPPPLQHYLE | QPVERVQRYQ | ALLKELIRNK | ||||
ARNRQNCALL | EQAYAVVSAL | PQRAENKLHV | SLMENYPGTL | QALGEPIRQG | ||||
HFIVWEGAPG | ARMPWKGHNR | HVFLFRNHLV | ICKPRRDSRT | DTVSYVFRNM | ||||
MKLSSIDLND | QVEGDDRAFE | VWQEREDSVR | KYLLQARTAI | IKSSWVKEIC | ||||
GIQQRLALPV | WRPPDFEEEL | ADCTAELGET | VKLACRVTGT | PKPVISWYKD | ||||
GKAVQVDPHH | ILIEDPDGSC | ALILDSLTGV | DSGQYMCFAA | SAAGNCSTLG | ||||
KILVQVPPRF | VNKVRASPFV | EGEDAQFTCT | IEGAPYPQIR | WYKDGALLTT | ||||
GNKFQTLSEP | RSGLLVLVIR | AASKEDLGLY | ECELVNRLGS | ARASAELRIQ | ||||
SPMLQAQEQC | HREQLVAAVE | DTTLERADQE | VTSVLKRLLG | PKAPGPSTGD | ||||
LTGPGPCPRG | APALQETGSQ | PPVTGTSEAP | AVPPRVPQPL | LHEGPEQEPE | ||||
AIARAQEWTV | PIRMEGAAWP | GAGTGELLWD | VHSHVVRETT | QRTYTYQAID | ||||
THTARPPSMQ | VTIEDVQAQT | GGTAQFEAII | EGDPQPSVTW | YKDSVQLVDS | ||||
TRLSQQQEGT | TYSLVLRHVA | SKDAGVYTCL | AQNTGGQVLC | KAELLVLGGD | ||||
NEPDSEKQSH | RRKLHSFYEV | KEEIGRGVFG | FVKRVQHKGN | KILCAAKFIP | ||||
LRSRTRAQAY | RERDILAALS | HPLVTGLLDQ | FETRKTLILI | LELCSSEELL | ||||
DRLYRKGVVT | EAEVKVYIQQ | LVEGLHYLHS | HGVLHLDIKP | SNILMVHPAR | ||||
EDIKICDFGF | AQNITPAELQ | FSQYGSPEFV | SPEIIQQNPV | SEASDIWAMG | ||||
VISYLSLTCS | SPFAGESDRA | TLLNVLEGRV | SWSSPMAAHL | SEDAKDFIKA | ||||
TLQRAPQARP | SAAQCLSHPW | FLKSMPAEEA | HFINTKQLKF | LLARSRWQRS | ||||
LMSYKSILVM | RSIPELLRGP | PDSPSLGVAR | HLCRDTGGSS | SSSSSSDNEL | ||||
APFARAKSLP | PSPVTHSPLL | HPRGFLRPSA | SLPEEAEASE | RSTEAPAPPA | ||||
SPEGAGPPAA | QGCVPRHSVI | RSLFYHQAGE | SPEHGALAPG | SRRHPARRRH | ||||
LLKGGYIAGA | LPGLREPLME | HRVLEEEAAR | EEQATLLAKA | PSFETALRLP | ||||
ASGTHLAPGH | SHSLEHDSPS | TPRPSSEACG | EAQRLPSAPS | GGAPIRDMGH | ||||
PQGSKQLPST | GGHPGTAQPE | RPSPDSPWGQ | PAPFCHPKQG | SAPQEGCSPH | ||||
PAVAPCPPGS | FPPGSCKEAP | LVPSSPFLGQ | PQAPPAPAKA | SPPLDSKMGP | ||||
GDISLPGRPK | PGPCSSPGSA | SQASSSQVSS | LRVGSSQVGT | EPGPSLDAEG | ||||
WTQEAEDLSD | STPTLQRPQE | QATMRKFSLG | GRGGYAGVAG | YGTFAFGGDA | ||||
GGMLGQGPMW | ARIAWAVSQS | EEEEQEEARA | ESQSEEQQEA | RAESPLPQVS | ||||
ARPVPEVGRA | PTRSSPEPTP | WEDIGQVSLV | QIRDLSGDAE | AADTISLDIS | ||||
EVDPAYLNLS | DLYDIKYLPF | EFMIFRKVPK | SAQPEPPSPM | AEEELAEFPE | ||||
PTWPWPGELG | PHAGLEITEE | SEDVDALLAE | AAVGRKRKWS | SPSRSLFHFP | ||||
GRHLPLDEPA | ELGLRERVKA | SVEHISRILK | GRPEGLEKEG | PPRKKPGLAS | ||||
FRLSGLKSWD | RAPTFLRELS | DETVVLGQSV | TLACQVSAQP | AAQATWSKDG | ||||
APLESSSRVL | ISATLKNFQL | LTILVVVAED | LGVYTCSVSN | ALGTVTTTGV | ||||
LRKAERPSSS | PCPDIGEVYA | DGVLLVWKPV | ESYGPVTYIV | QCSLEGGSWT | ||||
TLASDIFDCC | YLTSKLSRGG | TYTFRTACVS | KAGMGPYSSP | SEQVLLGGPS | ||||
HLASEEESQG | RSAQPLPSTK | TFAFQTQIQR | GRFSVVRQCW | EKASGRALAA | ||||
KIIPYHPKDK | TAVLREYEAL | KGLRHPHLAQ | LHAAYLSPRH | LVLILELCSG | ||||
PELLPCLAER | ASYSESEVKD | YLWQMLSATQ | YLHNQHILHL | DLRSENMIIT | ||||
EYNLLKVVDL | GNAQSLSQEK | VLPSDKFKDY | LETMAPELLE | GQGAVPQTDI | ||||
WAIGVTAFIM | LSAEYPVSSE | GARDLQRGLR | KGLVRLSRCY | AGLSGGAVAF | ||||
LRSTLCAQPW | GRPCASSCLQ | CPWLTEEGPA | CSRPAPVTFP | TARLRVFVRN | ||||
REKRRALLYK | RHNLAQVR |
Кодований геном білок за функціями належить до трансфераз, кіназ, cерин/треонінових протеїнкіназ, м'язових білків, білків розвитку, фосфопротеїнів. Задіяний у таких біологічних процесах як диференціація, поліморфізм, альтернативний сплайсинг. Білок має сайт для зв'язування з АТФ, нуклеотидами, молекулою кальмодуліну, іонами металів, іоном магнію. Локалізований у клітинній мембрані, цитоплазмі, ядрі, мембрані.
Література
- Young P.W., Ehler E., Gautel M. (2001). Obscurin, a giant sarcomeric Rho guanine nucleotide exchange factor protein involved in sarcomere assembly.. J. Cell Biol. 154: 123 — 136. PubMed DOI:10.1083/jcb.200102110
- Fukuzawa A., Idowu S., Gautel M. (2005). Complete human gene structure of obscurin: implications for isoform generation by differential splicing.. J. Muscle Res. Cell Motil. 26: 427 — 434. PubMed DOI:10.1007/s10974-005-9025-6
- Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O. (2000). Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.. DNA Res. 7: 273 — 281. PubMed DOI:10.1093/dnares/7.4.271
- Russell M.W., Raeker M.O., Korytkowski K.A., Sonneman K.J. (2002). Identification, tissue expression and chromosomal localization of human obscurin-MLCK, a member of the titin and Dbl families of myosin light chain kinases.. Gene 282: 237 — 246. PubMed DOI:10.1016/S0378-1119(01)00795-8
- Bagnato P., Barone V., Giacomello E., Rossi D., Sorrentino V. (2003). Binding of an ankyrin-1 isoform to obscurin suggests a molecular link between the sarcoplasmic reticulum and myofibrils in striated muscles.. J. Cell Biol. 160: 245 — 253. PubMed DOI:10.1083/jcb.200208109
Примітки
- Human PubMed Reference:.
- Mouse PubMed Reference:.
- HUGO Gene Nomenclature Commitee, HGNC:15719 (англ.). Процитовано 28 серпня 2017.
- UniProt, Q5VST9 (англ.). Процитовано 28 серпня 2017.
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