PRKDC
PRKDC (англ. Protein kinase, DNA-activated, catalytic polypeptide) – білок, який кодується однойменним геном, розташованим у людей на короткому плечі 8-ї хромосоми. [4] Довжина поліпептидного ланцюга білка становить 4 128 амінокислот, а молекулярна маса — 469 089[5].
Послідовність амінокислот
10 | 20 | 30 | 40 | 50 | ||||
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MAGSGAGVRC | SLLRLQETLS | AADRCGAALA | GHQLIRGLGQ | ECVLSSSPAV | ||||
LALQTSLVFS | RDFGLLVFVR | KSLNSIEFRE | CREEILKFLC | IFLEKMGQKI | ||||
APYSVEIKNT | CTSVYTKDRA | AKCKIPALDL | LIKLLQTFRS | SRLMDEFKIG | ||||
ELFSKFYGEL | ALKKKIPDTV | LEKVYELLGL | LGEVHPSEMI | NNAENLFRAF | ||||
LGELKTQMTS | AVREPKLPVL | AGCLKGLSSL | LCNFTKSMEE | DPQTSREIFN | ||||
FVLKAIRPQI | DLKRYAVPSA | GLRLFALHAS | QFSTCLLDNY | VSLFEVLLKW | ||||
CAHTNVELKK | AALSALESFL | KQVSNMVAKN | AEMHKNKLQY | FMEQFYGIIR | ||||
NVDSNNKELS | IAIRGYGLFA | GPCKVINAKD | VDFMYVELIQ | RCKQMFLTQT | ||||
DTGDDRVYQM | PSFLQSVASV | LLYLDTVPEV | YTPVLEHLVV | MQIDSFPQYS | ||||
PKMQLVCCRA | IVKVFLALAA | KGPVLRNCIS | TVVHQGLIRI | CSKPVVLPKG | ||||
PESESEDHRA | SGEVRTGKWK | VPTYKDYVDL | FRHLLSSDQM | MDSILADEAF | ||||
FSVNSSSESL | NHLLYDEFVK | SVLKIVEKLD | LTLEIQTVGE | QENGDEAPGV | ||||
WMIPTSDPAA | NLHPAKPKDF | SAFINLVEFC | REILPEKQAE | FFEPWVYSFS | ||||
YELILQSTRL | PLISGFYKLL | SITVRNAKKI | KYFEGVSPKS | LKHSPEDPEK | ||||
YSCFALFVKF | GKEVAVKMKQ | YKDELLASCL | TFLLSLPHNI | IELDVRAYVP | ||||
ALQMAFKLGL | SYTPLAEVGL | NALEEWSIYI | DRHVMQPYYK | DILPCLDGYL | ||||
KTSALSDETK | NNWEVSALSR | AAQKGFNKVV | LKHLKKTKNL | SSNEAISLEE | ||||
IRIRVVQMLG | SLGGQINKNL | LTVTSSDEMM | KSYVAWDREK | RLSFAVPFRE | ||||
MKPVIFLDVF | LPRVTELALT | ASDRQTKVAA | CELLHSMVMF | MLGKATQMPE | ||||
GGQGAPPMYQ | LYKRTFPVLL | RLACDVDQVT | RQLYEPLVMQ | LIHWFTNNKK | ||||
FESQDTVALL | EAILDGIVDP | VDSTLRDFCG | RCIREFLKWS | IKQITPQQQE | ||||
KSPVNTKSLF | KRLYSLALHP | NAFKRLGASL | AFNNIYREFR | EEESLVEQFV | ||||
FEALVIYMES | LALAHADEKS | LGTIQQCCDA | IDHLCRIIEK | KHVSLNKAKK | ||||
RRLPRGFPPS | ASLCLLDLVK | WLLAHCGRPQ | TECRHKSIEL | FYKFVPLLPG | ||||
NRSPNLWLKD | VLKEEGVSFL | INTFEGGGCG | QPSGILAQPT | LLYLRGPFSL | ||||
QATLCWLDLL | LAALECYNTF | IGERTVGALQ | VLGTEAQSSL | LKAVAFFLES | ||||
IAMHDIIAAE | KCFGTGAAGN | RTSPQEGERY | NYSKCTVVVR | IMEFTTTLLN | ||||
TSPEGWKLLK | KDLCNTHLMR | VLVQTLCEPA | SIGFNIGDVQ | VMAHLPDVCV | ||||
NLMKALKMSP | YKDILETHLR | EKITAQSIEE | LCAVNLYGPD | AQVDRSRLAA | ||||
VVSACKQLHR | AGLLHNILPS | QSTDLHHSVG | TELLSLVYKG | IAPGDERQCL | ||||
PSLDLSCKQL | ASGLLELAFA | FGGLCERLVS | LLLNPAVLST | ASLGSSQGSV | ||||
IHFSHGEYFY | SLFSETINTE | LLKNLDLAVL | ELMQSSVDNT | KMVSAVLNGM | ||||
LDQSFRERAN | QKHQGLKLAT | TILQHWKKCD | SWWAKDSPLE | TKMAVLALLA | ||||
KILQIDSSVS | FNTSHGSFPE | VFTTYISLLA | DTKLDLHLKG | QAVTLLPFFT | ||||
SLTGGSLEEL | RRVLEQLIVA | HFPMQSREFP | PGTPRFNNYV | DCMKKFLDAL | ||||
ELSQSPMLLE | LMTEVLCREQ | QHVMEELFQS | SFRRIARRGS | CVTQVGLLES | ||||
VYEMFRKDDP | RLSFTRQSFV | DRSLLTLLWH | CSLDALREFF | STIVVDAIDV | ||||
LKSRFTKLNE | STFDTQITKK | MGYYKILDVM | YSRLPKDDVH | AKESKINQVF | ||||
HGSCITEGNE | LTKTLIKLCY | DAFTENMAGE | NQLLERRRLY | HCAAYNCAIS | ||||
VICCVFNELK | FYQGFLFSEK | PEKNLLIFEN | LIDLKRRYNF | PVEVEVPMER | ||||
KKKYIEIRKE | AREAANGDSD | GPSYMSSLSY | LADSTLSEEM | SQFDFSTGVQ | ||||
SYSYSSQDPR | PATGRFRRRE | QRDPTVHDDV | LELEMDELNR | HECMAPLTAL | ||||
VKHMHRSLGP | PQGEEDSVPR | DLPSWMKFLH | GKLGNPIVPL | NIRLFLAKLV | ||||
INTEEVFRPY | AKHWLSPLLQ | LAASENNGGE | GIHYMVVEIV | ATILSWTGLA | ||||
TPTGVPKDEV | LANRLLNFLM | KHVFHPKRAV | FRHNLEIIKT | LVECWKDCLS | ||||
IPYRLIFEKF | SGKDPNSKDN | SVGIQLLGIV | MANDLPPYDP | QCGIQSSEYF | ||||
QALVNNMSFV | RYKEVYAAAA | EVLGLILRYV | MERKNILEES | LCELVAKQLK | ||||
QHQNTMEDKF | IVCLNKVTKS | FPPLADRFMN | AVFFLLPKFH | GVLKTLCLEV | ||||
VLCRVEGMTE | LYFQLKSKDF | VQVMRHRDDE | RQKVCLDIIY | KMMPKLKPVE | ||||
LRELLNPVVE | FVSHPSTTCR | EQMYNILMWI | HDNYRDPESE | TDNDSQEIFK | ||||
LAKDVLIQGL | IDENPGLQLI | IRNFWSHETR | LPSNTLDRLL | ALNSLYSPKI | ||||
EVHFLSLATN | FLLEMTSMSP | DYPNPMFEHP | LSECEFQEYT | IDSDWRFRST | ||||
VLTPMFVETQ | ASQGTLQTRT | QEGSLSARWP | VAGQIRATQQ | QHDFTLTQTA | ||||
DGRSSFDWLT | GSSTDPLVDH | TSPSSDSLLF | AHKRSERLQR | APLKSVGPDF | ||||
GKKRLGLPGD | EVDNKVKGAA | GRTDLLRLRR | RFMRDQEKLS | LMYARKGVAE | ||||
QKREKEIKSE | LKMKQDAQVV | LYRSYRHGDL | PDIQIKHSSL | ITPLQAVAQR | ||||
DPIIAKQLFS | SLFSGILKEM | DKFKTLSEKN | NITQKLLQDF | NRFLNTTFSF | ||||
FPPFVSCIQD | ISCQHAALLS | LDPAAVSAGC | LASLQQPVGI | RLLEEALLRL | ||||
LPAELPAKRV | RGKARLPPDV | LRWVELAKLY | RSIGEYDVLR | GIFTSEIGTK | ||||
QITQSALLAE | ARSDYSEAAK | QYDEALNKQD | WVDGEPTEAE | KDFWELASLD | ||||
CYNHLAEWKS | LEYCSTASID | SENPPDLNKI | WSEPFYQETY | LPYMIRSKLK | ||||
LLLQGEADQS | LLTFIDKAMH | GELQKAILEL | HYSQELSLLY | LLQDDVDRAK | ||||
YYIQNGIQSF | MQNYSSIDVL | LHQSRLTKLQ | SVQALTEIQE | FISFISKQGN | ||||
LSSQVPLKRL | LNTWTNRYPD | AKMDPMNIWD | DIITNRCFFL | SKIEEKLTPL | ||||
PEDNSMNVDQ | DGDPSDRMEV | QEQEEDISSL | IRSCKFSMKM | KMIDSARKQN | ||||
NFSLAMKLLK | ELHKESKTRD | DWLVSWVQSY | CRLSHCRSRS | QGCSEQVLTV | ||||
LKTVSLLDEN | NVSSYLSKNI | LAFRDQNILL | GTTYRIIANA | LSSEPACLAE | ||||
IEEDKARRIL | ELSGSSSEDS | EKVIAGLYQR | AFQHLSEAVQ | AAEEEAQPPS | ||||
WSCGPAAGVI | DAYMTLADFC | DQQLRKEEEN | ASVIDSAELQ | AYPALVVEKM | ||||
LKALKLNSNE | ARLKFPRLLQ | IIERYPEETL | SLMTKEISSV | PCWQFISWIS | ||||
HMVALLDKDQ | AVAVQHSVEE | ITDNYPQAIV | YPFIISSESY | SFKDTSTGHK | ||||
NKEFVARIKS | KLDQGGVIQD | FINALDQLSN | PELLFKDWSN | DVRAELAKTP | ||||
VNKKNIEKMY | ERMYAALGDP | KAPGLGAFRR | KFIQTFGKEF | DKHFGKGGSK | ||||
LLRMKLSDFN | DITNMLLLKM | NKDSKPPGNL | KECSPWMSDF | KVEFLRNELE | ||||
IPGQYDGRGK | PLPEYHVRIA | GFDERVTVMA | SLRRPKRIII | RGHDEREHPF | ||||
LVKGGEDLRQ | DQRVEQLFQV | MNGILAQDSA | CSQRALQLRT | YSVVPMTSRL | ||||
GLIEWLENTV | TLKDLLLNTM | SQEEKAAYLS | DPRAPPCEYK | DWLTKMSGKH | ||||
DVGAYMLMYK | GANRTETVTS | FRKRESKVPA | DLLKRAFVRM | STSPEAFLAL | ||||
RSHFASSHAL | ICISHWILGI | GDRHLNNFMV | AMETGGVIGI | DFGHAFGSAT | ||||
QFLPVPELMP | FRLTRQFINL | MLPMKETGLM | YSIMVHALRA | FRSDPGLLTN | ||||
TMDVFVKEPS | FDWKNFEQKM | LKKGGSWIQE | INVAEKNWYP | RQKICYAKRK | ||||
LAGANPAVIT | CDELLLGHEK | APAFRDYVAV | ARGSKDHNIR | AQEPESGLSE | ||||
ETQVKCLMDQ | ATDPNILGRT | WEGWEPWM |
Кодований геном білок за функціями належить до трансфераз, кіназ, cерин/треонінових протеїнкіназ. Задіяний у таких біологічних процесах як пошкодження ДНК, репарація ДНК, біологічні ритми, рекомбінація ДНК. Білок має сайт для зв'язування з АТФ, нуклеотидами, ДНК. Локалізований у ядрі.
Література
- Anderson C.W., Dunn J.J., Freimuth P.I., Galloway A.M., Allalunis-Turner M.J. (2001). Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J.. Radiat. Res. 156: 2 — 9. PubMed DOI:10.1667/0033-7587(2001)156[0002:FMIPTG]2.0.CO;2
- Carter T., Vancurova I., Sun I., Lou W., DeLeon S. (1990). A DNA-activated protein kinase from HeLa cell nuclei.. Mol. Cell. Biol. 10: 6460 — 6471. PubMed DOI:10.1128/MCB.10.12.6460
- Iijima S., Teraoka H., Date T., Tsukada K. (1992). DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein.. Eur. J. Biochem. 206: 595 — 603. PubMed DOI:10.1111/j.1432-1033.1992.tb16964.x
- Bannister A.J., Gottlieb T.M., Kouzarides T., Jackson S.P. (1993). c-Jun is phosphorylated by the DNA-dependent protein kinase in vitro; definition of the minimal kinase recognition motif.. Nucleic Acids Res. 21: 1289 — 1295. PubMed DOI:10.1093/nar/21.5.1289
- Connelly M.A., Zhang H., Kieleczawa J., Anderson C.W. (1996). Alternate splice-site utilization in the gene for the catalytic subunit of the DNA-activated protein kinase, DNA-PKcs.. Gene 175: 271 — 273. PubMed DOI:10.1016/0378-1119(96)00135-7
- Shieh S.-Y., Ikeda M., Taya Y., Prives C. (1997). DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2.. Cell 91: 325 — 334. PubMed DOI:10.1016/S0092-8674(00)80416-X
Примітки
- Захворювання, генетично пов'язані з PRKDC переглянути/редагувати посилання на ВікіДаних.
- Human PubMed Reference:.
- Mouse PubMed Reference:.
- HUGO Gene Nomenclature Commitee, HGNC:9413 (англ.). Процитовано 6 лютого 2017.
- UniProt, P78527 (англ.). Процитовано 6 лютого 2017.
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